Equine cartilage secretome 5 days IL-1 treatment in vitro;'Neopeptide Analyser' output files

Neopeptide Analyser output files.

Experiment n=8 equine metacarpophalangeal cartilage explants from grossly normal joints treated in vitro with 10ng/ml IL-1 for 5 days. Secretome samples collected and pooled for 5 days. Media samples tryspin digested and run on an Orbitrap-Velos mass spectrometer. Raw files input into Progenesis QIP and label-free quantification undertaken with Mascot used to search for semi-tryptic peptides. Peptide measurements output file from ProgenesisQIP is then used as the  input file into the 'Neopeptide Analyser' for neopeptide identification. The files here are the output files from the 'Neopeptide Analyser'. They are suffixed _filter and _processed.