Env adaptations partly inhibit the capacity of packaged A3G to deaminate viral cDNA.

(A) Immunoblots of viral particles of the indicated genotypes produced in SupT11-A3G cells and fractionated by ultracentrifugation through a sucrose gradient in the presence or absence of detergent. A3G co-sediments with viral core components under all conditions except the Vif-proficient scenario where it is efficiently degraded. (B) Representative images of pol 3D-PCR products using viral cDNAs subjected to ERT. The indicated viruses were originally produced in SupT11 cells expressing A3G. (C) G-to-A mutation loads of high-fidelity, high temperature pol gene amplicons from viruses originally produced in SupT11-A3G and subjected to ERT (mean +/- SD of independent 4 experiments with a minimum of 10 sequences or 5,640 bp analyzed per condition). Statistical comparisons were done using a one-way ANOVA and Fisher’s LSD test (p-values above each panel).