Enhanced bacoside production in shoot cultures of <i>Bacopa monnieri</i> under the influence of abiotic elicitors

<div><p>The effect of different abiotic elicitors [jasmonic acid, copper sulphate (CuSO<sub>4</sub>) and salicyclic acid] at varying concentrations on the stimulation of biomass and bacoside production in <i>in vitro Bacopa monnieri</i> shoot culture was studied. A systematic study conducted over a period of 35 days indicated that the maximum bacoside production (6.74 mg g<sup>− 1</sup> dry weight (DW)) was obtained after a lag of 7 days and thereafter, the content decreased gradually to again increase at 28 days (5.91 mg g<sup>− 1</sup> DW). Therefore, elicitation experiments were carried out over a period of 3, 6 and 9 days. The shoot cultures treated with 45 mg L<sup>− 1</sup> of CuSO<sub>4</sub> exhibited the highest bacoside content of 8.73 mg g<sup>− 1</sup> DW (∼1.42-fold higher) than in control cultures (6.14 mg g<sup>− 1</sup> DW). This study indicates the effectiveness of abiotic elicitation on bacoside production in <i>in vitro</i> shoot cultures of this medicinally important herb known for its memory-enhancing properties.</p></div>