nl7b01382_si_001.pdf (9.84 MB)
Enhanced Phototherapy by Nanoparticle-Enzyme via Generation and Photolysis of Hydrogen Peroxide
journal contribution
posted on 2017-06-14, 00:00 authored by Kaiwen Chang, Zhihe Liu, Xiaofeng Fang, Haobin Chen, Xiaoju Men, Ye Yuan, Kai Sun, Xuanjun Zhang, Zhen Yuan, Changfeng WuLight has been widely
used for cancer therapeutics such as photodynamic therapy (PDT) and
photothermal therapy. This paper describes a strategy called enzyme-enhanced
phototherapy (EEPT) for cancer treatment. We constructed a nanoparticle
platform by covalent conjugation of glucose oxidase (GOx) to small
polymer dots, which could be persistently immobilized into a tumor.
While the malignant tumors have high glucose uptake, the GOx efficiently
catalyzes the glucose oxidation with simultaneous generation of H2O2. Under light irradiation, the in situ generated
H2O2 was photolyzed to produce hydroxyl radical,
the most reactive oxygen species, for killing cancer cells. In vitro
assays indicated that the cancer cells were destroyed by using a nanoparticle
concentration at 0.2 μg/mL and a light dose of ∼120 J/cm2, indicating the significantly enhanced efficiency of the
EEPT method when compared to typical PDT that requires a photosensitizer
of >10 μg/mL for effective cell killing under the same light
dose. Furthermore, remarkable inhibition of tumor growth was observed
in xenograft-bearing mice, indicating the promise of the EEPT approach
for cancer therapeutics.