posted on 2017-11-09, 15:45authored byJuhua Liu, Jing Zhang, Hongxia Miao, Caihong Jia, Jingyi Wang, Biyu Xu, Zhiqiang Jin
The ovate mutation has frequently been used to
study changes in fruit shape but not fruit quality. A deterioration
in fruit quality associated with the ovate mutation
was discovered in this study. To elucidate how ovate influences the quality of fruit, we performed a proteomics analysis
of the fruits of the ovate mutant (LA3543) and wild-type
(“Ailsa Craig”, LA2838A) using tandem mass tag analysis.
The results indicated that the ovate mutation significantly
influences fruit quality in a number of ways, including by reducing
the expression of 1-aminocyclopropane-1-carboxylic acid oxidase 3
(ACO3) in ethylene biosynthesis, improving firmness by reducing the
amount of pectinesterase and polygalacturonase, reducing sugar accumulation
by downregulating the abundance of mannan endo-1,4-β-mannosidase
4, β-galactosidase, and β-amylase, and reducing the malic
acid content by downregulating the accumulation of malic enzymes and
malate synthase. These findings could inform future improvements in
fruit quality.