Electron microscopic evidence of colocalization of exogenous HIV-1 VLPs and endogenous mature virions within the VCC in HIV-1-infected MDMs.

<p><b>(A)</b> 400 ng of HIV-1 Gag-EGFP VLPs (ratio of WT:Gag-EGFP 1:1) were added to mature MDMs and allowed to internalize overnight prior to harvest and subsequent processing for transmission EM. <b>(B)</b> Enlargement of white dotted boxed area from <b>(A)</b>. <b>(C)</b> NLUdel infected MDMs at day 10 post-infection displaying typical VCC morphology incorporating large amounts of mature HIV-1. <b>(D)</b> Enlargement of white dotted boxed area from <b>(C)</b>. <b>(E)</b> MDMs were infected with NLUdel at a TCID50 of 2.0/cell. On day 9 post-infection, 400 ng of HIV-1 Gag-EGFP VLPs (ratio of WT:Gag-EGFP 3:1) were allowed to internalize overnight prior to harvest and processing. <b>(F)</b> Enlargement of white dotted boxed area from <b>(E)</b>. <b>(G-L)</b> Same procedure as <b>(E)</b>, except HIV-1 Gag-EGFP VLPs produced at a WT:Gag-EGFP ratio of 1:1 in order to simplify identification of exogenous VLPs. In each paired image, the enlarged section is indicated by a white broken line in the preceding image. Images were acquired on a 120 kV Hitachi H-7500 transmission electron microscope. Size bars = 500 nm.</p>