SMP_runall_Map_meta_FINAL2017.txt (20.29 kB)
Effects of host species and environment on the skin microbiome of Plethodontid salamanders
dataset
posted on 2017-05-23, 14:04 authored by Carly Muletz WolzCarly Muletz Wolz, Karen LipsKaren Lips, Stephanie Yarwood, Evan Campbell Grant, Robert FleischerEnvironmental metadata for 100 Plethodon salamanders of three species sampled in the Central Appalachian Mountains. We conducted a general descriptive analysis of the bacterial OTUs identified from
the three host species (n = 100), including description of their core microbiome.
Then, we examined microbiome structure in two subsets of the data: a
co-occurring species dataset (n = 62) and an elevational dataset (n = 50). Twelve individuals were included in both analyses.
We
characterized skin microbiome structure (alpha-diversity, beta-diversity and bacterial operational
taxonomic unit [OTU] abundances) using 16S rRNA gene sequencing for
co-occurring Plethodon salamander species (35 P. cinereus, 17 P.
glutinosus, 10 P. cylindraceus) at three localities to differentiate the effects of
host species from environmental factors on the microbiome. We sampled the
microbiome of P. cinereus along an elevational gradient (n = 50, 700 – 1000 masl) at one
locality to determine whether elevation predicts microbiome structure. Finally,
we quantified prevalence and abundance of putatively anti-Bd bacteria to
determine if Bd-inhibitory bacteria are dominant microbiome members.
Pyrosequencing runs have been deposited in the
National Center for Biotechnology Information Sequence Read Archive (www.ncbi.nlm.nih.gov/sra)
under accession number SUB2660574
