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Effects of heap-bioleaching plant on physicochemical index and microbial community of the nearby river
The Zijinshan
heap-bioleaching plant started the operation by the end of 2005, since a river
named Ting River near the heap-bioleaching system, there was raising concern
about the acidophiles and metal ion migration into this river. In this
research, 72 liquid samples and
9 ore heap samples were collected, the distribution
of acidophiles in Ting River were investigated by clone libraries, two key
acidophiles included genus Acidithiobacillus,
Leptospirillum were assayed by
real-time polymerase chain reaction, and physicochemical
characteristics of the samples were analyzed. The clone libraries results
showed that 1291 OTUs were got from 3669 sequences, and these 1291 OTUs
belonged to 16 phyla and 166 genera. There was more community structure similar
between the samples of 2012 year and 2013 year. The detected bioleaching
microorganisms emerged the obvious changing trend of increasing in the period
2009 to 2012, and emerged the decreasing trend in the period 2012 to 2013 on
whole. During the period 2009 to 2013, the dissolved copper emerged the
decreasing trend, but the dissolved iron emerged the increased trend on the
whole. The changes trend of dissolved copper and iron did not relate with the
bioleaching microbes.