Effects of coconut oil rescue on Pex19 mutants’ fatty acid profile and neurodegeneration.

(A) FAMEs comparing w- and Pex19−/− fed with control and M/LCFA-enriched rescue food. Absolute concentration in nmol/mg wet tissue. n = 7. Significance tested with ANOVA. (B) Calculation of the average chain length from the absolute FAME values. (C) Larval locomotion assay as a readout for neurodegeneration. Dots represent single experiments, bars represent median. Significance tested with Student t test. (D-G) Annexin V-FITC staining of 1-day-old adult brains. Scale bars represent 100 μm. n = 5. Error bars represent SD. *p < 0.05; **p < 0.01; ***p < 0.001. Corresponding raw data can be found in supplemental file S1 Data. FAME, fatty acid methyl ester; FITC, fluorescein isothiocyanate; M/LCFA, medium- and long-chain fatty acid; ns, not significant.