Effect of Leptospira infection on tight junction and gap junction proteins in endothelial cells.

(A) tight junction protein, zonula occludens-1 (ZO-1) and (B) gap junction protein, connexin 43 (connexin) in HDLEC are shown in green. The nuclei are stained in blue for all panels. Scale bars represent 50 μm. Quantified signal intensity of the host protein is indicated in a right-hand graph (mean +/- SD, p-value is indicated below each graph, the independent p-value shown as an asterisk for Copenhageni is compared to uninfected and Patoc-infected cells).