Effect of PU.1 knockdown by siRNA on the mRNA levels of CIITA and MHC class II in mouse pDCs and human pDC cell line.

The mRNA expression levels of PU.1 (A, E), MHC class II (B, F), CIITA (C, G), and CIITA specifically driven from pIII (D, H) in mouse PU.1 siRNA-introduced BMpDCs (A-D), or human PU.1 siRNA (Spi1-HSS186060)-introduced CAL-1 cells (E-H) are displayed as the ratio of mRNA levels versus those seen in control siRNA-introduced cells. The mRNA levels of PU.1 (I) and CIITA (J) in CAL-1 cells transfected with another PU.1 siRNA (Spi1-HSS144058) are also displayed. Cells were harvested at 24h (A-D) or 96h (E-J) after siRNA transfection. Open bar, PU.1 siRNA; closed bar, control siRNA. In E-J, data represent means ± SEMs of three independent experiments, and each experiment was performed with triplicate samples. In A-E, the results are expressed as means ± SEMs of triplicate samples, and similar result was obtained in another experiment. *, p < 0.05.