ES induces p-CREB in DRGNs.

(A) p-CREB localization in cultured DRGNs with or without ES. p-CREB (green) was localized in the nuclei. β-tubulin III (red) indicated cell bodies and neurites. Double staining was performed in Shams (a, b, c) and cells with 30 min ES (d, e, f). Scale bar (50 μm) applies to a—f. (B) p-CREB expression in cells with 0–60 min ES (mean ± SD; n = 3). Histone expression was used as a loading control.