Fig 2.TIF (812.57 kB)
EBNA3C is an important contributor to the enhanced expression of E2F6.
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posted on 2016-08-22, 04:30 authored by Yonggang Pei, Shuvomoy Banerjee, Zhiguo Sun, Hem Chandra Jha, Abhik Saha, Erle S. RobertsonA-C) 10 million EBV-negative BJAB, Ramos, EBNA3C stable expressing BJAB7, BJAB10, EBV-transformed LCL1, LCL2, and sh-ctrl (stable control knockdown), sh-EBNA3C (stable EBNA3C knockdown) LCL1 cells were harvested and lysed. Western blot analysis of E2F6 protein expression levels was shown with indicated antibodies. D-E) 10 million Ramos or HEK-293 cells were co-transfected with increasing doses of EBNA3C expressing constructs (0, 5, 10, 15, 20 μg) and E2F6, EBNA3C, GAPDH proteins were detected with western blot analysis. GAPDH was used as an internal loading control.
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E 2F transcription factorsEBNA 3C cell proliferationE 2F promoterE 2F expressionantigen 3 CEBNA 3CE 2F bindingLCLInhibit E 2F TranscriptionE 2F genesDNAcarboxy terminal domainsE 2F formsE 2F transcriptional activityE 2F transcription regulatorE 2F family membersE 2F proteinE 2F activitiesEBV Nuclear Antigen 3 C Mediates RegulationE 2F
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