EBNA3C is an important contributor to the enhanced expression of E2F6.
A-C) 10 million EBV-negative BJAB, Ramos, EBNA3C stable expressing BJAB7, BJAB10, EBV-transformed LCL1, LCL2, and sh-ctrl (stable control knockdown), sh-EBNA3C (stable EBNA3C knockdown) LCL1 cells were harvested and lysed. Western blot analysis of E2F6 protein expression levels was shown with indicated antibodies. D-E) 10 million Ramos or HEK-293 cells were co-transfected with increasing doses of EBNA3C expressing constructs (0, 5, 10, 15, 20 μg) and E2F6, EBNA3C, GAPDH proteins were detected with western blot analysis. GAPDH was used as an internal loading control.