Dominant repressive H3K27me3 histone modifications at the RTA promoter region in classic KS tissues.

(A) Illustration of AcH3 and H3K27me3 histone modifications at RTA promoter region (HQ404500: 68000–71000). The signals of AcH3 and H3K27 histone modifications were overlaid with the signals of Input (baseline) respectively. Red: AcH3 or H3K27. Blue: Input. *: the region of relative strong H3K27me3 enrichment. (B) Results of ChIP-qPCR at the region of RTA promoter and GAPDH gene (control) in classic KS tissues. Samples prepared for ChIP-Seq were divided and a small quantity (1/5) kept for ChIP-qPCR assay before library construction. ChIP–qPCR data were normalized by the percent input method (signals obtained from ChIP were divided by signals obtained from an input sample). Data are presented as mean±SD. N.A. represents no amplification.