Version 2 2020-04-23, 06:44Version 2 2020-04-23, 06:44
Version 1 2020-03-28, 02:58Version 1 2020-03-28, 02:58
dataset
posted on 2020-04-23, 06:44authored byFabio ZaniniFabio Zanini, Racquel Domingo-Gonzalez, Xibing Che, Robert C Jones, Michael A Swift, Stephen R Quake, David N Cornfield, Cristina M Alvira
At birth, the lungs experience a sudden transition from a pathogen-free,
hypoxic, fluid-filled environment to a pathogen-rich, rhythmically
distended air-liquid interface. While many studies focus on adult
tissue, the heterogeneity of immune cells in the perinatal lung remains
unexplored. Here, we combine single cell transcriptomics with in situ
hybridization to present an atlas of the murine lung immune compartment
during a critical period of lung development. We show that the late
embryonic lung is dominated by specialized proliferative macrophages
with a surprising physical interaction with the developing vasculature.
These macrophages disappear after birth and are replaced by a complex
and dynamic mixture of macrophage subtypes, dendritic cells,
granulocytes, and lymphocytes. Detailed characterization of macrophage
diversity revealed a precise orchestration of five distinct
subpopulations across postnatal development to fill context-specific
functions in tissue remodeling, angiogenesis, and immunity. These data
both broaden the putative roles for immune cells in the developing lung
and provide a framework for understanding how external insults alter
immune cell phenotype during a period of rapid lung growth and
heightened vulnerability.
File formats:
- tsv (gzipped): separate files for gene expression counts and cell metadata
- loom: single file with both cell metadata and gene expression counts