Distribution of operon types in the genome and frequency of transcriptional features within non-traditional operons identified using StringTie.

(A) The pie chart describes the distribution of the types of operons present in T4 annotated using only high confidence TSSs. A total of 388 multigene and 343 single gene operons were identified, which can be divided up in 47% simple operons (single gene transcriptional units with a single TSS and TTS; gray), 26% complex operons (multi-gene operons with multiple TSSs and TTSs; orange), 10% traditional operons (multi-gene operon with a single TSS and TTS; green), 1% multiTSS operons (blue), and 16% multiTTS operons (red). (B) The clustered histogram describes the distribution of genes and transcriptional features in non-traditional operons, where gray represents the numbers of genes in the multigene operons, green represents the number of TSSs within operons, red represents the number of TTSs within operons. Two-gene operons are found most frequently in the non-traditional operons with one internal TSS and TTS.