Distribution of SOD isoforms and Pchlide fluorescence along etiolated wheat leaves.

A) Experimental model: tip (T), middle (M) and basal (B) leaf fragments were collected for the experiments; B) Native PAGE for the detection of SOD in T, M and B leaf fragments; the pooled sample was used for electrophoresis. Each well was loaded with 25 μg of protein; C) The distribution of the relative activity of SOD isoforms in B, M and T etiolated leaf fragments; the data represents the mean ± SD, n = 3, * p < 0.05, ** p < 0.01; D) 77 K fluorescence emission spectra of T, M and B etiolated leaf fragments; excitation: 440 nm; E) The average ratio of fluorescence intensity at 654 nm to the intensity at 633 nm (F654/F633) read from fluorescence spectra measured for single leaf fragments, the data represents mean ± SD, n > 30, *** p < 0.001.