Developmental Brain Protein Level Changes in the C57BL/6J Mouse

As work on systematic searches for developmentally-regulated proteins is limited, whole brains of CL57B/6J mice were taken from day 16 of gestation, at postnatal days 7 and 54−58. Proteins were extracted, and analyzed by a gel-based proteomic method (ion trap HCT). Antioxidant, cytoskeleton, chaperone, several metabolic proteins from different pathways, proteins from the protein synthetic and degradation machinery, sirtuin and a mitochondrial inner membrane protein were developmentally regulated.