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Development of a Paper-Based Analytical Device for Colorimetric Detection of Select Foodborne Pathogens
journal contribution
posted on 2012-03-20, 00:00 authored by Jana C. Jokerst, Jaclyn
A. Adkins, Bledar Bisha, Mallory M. Mentele, Lawrence D. Goodridge, Charles S. HenryFoodborne pathogens are a major public health threat
and financial
burden for the food industry, individuals, and society, with an estimated
76 million cases of food-related illness occurring in the United States
alone each year. Three of the most important causative bacterial agents
of foodborne diseases are pathogenic strains of Escherichia
coli, Salmonella spp.,
and Listeria monocytogenes, due to
the severity and frequency of illness and disproportionally high number
of fatalities. Their continued persistence in food has dictated the
ongoing need for faster, simpler, and less expensive analytical systems
capable of live pathogen detection in complex samples. Culture techniques
for detection and identification of foodborne pathogens require 5–7
days to complete. Major improvements to molecular detection techniques
have been introduced recently, including polymerase chain reaction
(PCR). These methods can be tedious; require complex, expensive instrumentation;
necessitate highly trained personnel; and are not easily amenable
to routine screening. Here, a paper-based analytical device (μPAD)
has been developed for the detection of E. coli O157:H7, Salmonella Typhimurium,
and L. monocytogenes in food samples
as a screening system. In this work, a paper-based microspot assay
was created by use of wax printing on filter paper. Detection is achieved
by measuring the color change when an enzyme associated with the pathogen
of interest reacts with a chromogenic substrate. When combined with
enrichment procedures, the method allows for an enrichment time of
12 h or less and is capable of detecting bacteria in concentrations
in inoculated ready-to-eat (RTE) meat as low as 101 colony-forming
units/cm2.
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Select Foodborne PathogensFoodborne pathogensfood samplesColorimetric DetectionPADfoodborne diseasesculture techniquesmethoddetection techniquesPCRRTESalmonellaUnited Statespathogen detectionfilter paperscreening systemEscherichia colienrichment proceduresenrichment timepolymerase chain reactionfood industryillnesshealth threatwax printingchromogenic substrateMajor improvementsfoodborne pathogens12 hcolor change
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