mz7b00242_si_001.pdf (297.09 kB)
Development of Excipient-Free Freeze-Dryable Unimolecular Hyperstar Polymers for Efficient siRNA Silencing
journal contribution
posted on 2017-06-19, 13:05 authored by Liang Zhao, Xiang Wu, Xiaofeng Wang, Chunying Duan, Hongyan Wang, Amol Punjabi, Yang Zhao, Yuanwei Zhang, Zuoshang Xu, Haifeng Gao, Gang HanWe
designed a unimolecular hyperstar polymer for efficient small
interfering RNA (siRNA) delivery that can be processed under repeated
lyophilization and reconstitution without the need of any excipient.
The hyperstar polymer contains a biodegradable hyperbranched core
and is bound to siRNA through its thousands of cationic arms that
radiate from its core. The siRNA/hyperstar complexes showed siRNA
transfection efficiency that was superior to that of Lipofectamine2000
in regard to the gene for human Cu, Zn superoxide dismutase 1 (SOD1),
whose mutation causes familial amyotrophic lateral sclerosis. More
importantly, hyperstar polymers as unimolecular containers minimized
the multipolymer cross-interaction during lyophilization, and this
maintained the uniquely high transfection efficiency of the siRNA/hyperstar
complexes after repeated freeze-drying and reconstitution without
the conventional need for excipient.
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hyperbranched coretransfection efficiencyhyperstar polymerExcipient-Free Freeze-Dryable Unimolecular Hyperstar PolymersLipofectamine 2000siRNA transfection efficiencyZn superoxide dismutase 1unimolecular hyperstar polymerSODmultipolymer cross-interactionEfficient siRNA Silencingmutation causescationic armsunimolecular containershyperstar polymers
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