gpom_a_1354193_sm7041.docx (123.8 kB)
Development of docetaxel-loaded PEG–PLA nanoparticles using surfactant-free method for controlled release studies
Version 2 2017-08-29, 13:40
Version 1 2017-07-19, 14:39
journal contribution
posted on 2017-08-29, 13:40 authored by Prajna Mishra, Ratan Kumar DeyDocetaxel (DTX)-loaded poly(ethylene glycol)–poly(D,L-lactide) is prepared by nanoprecipitation method in the absence of any surfactants. The average particle size of the copolymer was found to be 101 nm. The drug entrapment efficiency (%) and drug loading (%) of polymer were found to be 9.471 ± 0.047 and 94.71 ± 0.466, respectively. The in vitro drug release characteristics show the controlled release of 98% of docetaxel in 72 h. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and apoptosis measured in terms of cleaved Poly(ADP-ribose) Polymerase (PARP) and cleaved caspase-3 protein expression shows that the copolymer has better cytotoxicity effect and apoptosis in comparison to free DTX in HeLa cells.