Determination of the cell cycle using image and flow cytometry.

<p><b>(A)</b> The bivariate analysis of the replication signal and DNA content in HeLa cells after a 30-minute incubation with 10 μM BrdU using image cytometry. DNase I and exonuclease III were used for BrdU detection in DNA. (<b>B)</b> The bivariate analysis of the replication signal and DNA content in A549 cells after a 30-minute incubation with 10 μM BrdU using flow cytometry. DNase I and exonuclease III were used for the BrdU detection in DNA.</p>