Fig 5.tif (310.98 kB)
Determination of the cell cycle using image and flow cytometry.
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posted on 2017-03-30, 18:12 authored by Anna Ligasová, Petr Konečný, Ivo Frydrych, Karel Koberna(A) The bivariate analysis of the replication signal and DNA content in HeLa cells after a 30-minute incubation with 10 μM BrdU using image cytometry. DNase I and exonuclease III were used for BrdU detection in DNA. (B) The bivariate analysis of the replication signal and DNA content in A549 cells after a 30-minute incubation with 10 μM BrdU using flow cytometry. DNase I and exonuclease III were used for the BrdU detection in DNA.
