Detection of homogentisate from <i>Ralstonia solanacearum</i> strains by high-performance liquid chromatography.

<p>HPLC analysis of the culture filtrate of wild-type SL341 and SL341D over time for the detection of homogentisate (HGA), an intermediate in pyomelanin synthesis. (A, B) Tyrosine standard and HGA standard peaks. (C, D) Culture filtrate analysis of SL341 after 48 and 72 h growth, respectively. (E, F) Culture filtrate analysis of SL341D after 48 and 72 h growth, respectively.</p>