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Detection of homogentisate from Ralstonia solanacearum strains by high-performance liquid chromatography.

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posted on 2016-08-11, 17:44 authored by Shabir Ahmad, Seung Yeup Lee, Hyun Gi Kong, Eun Jeong Jo, Hye Kyung Choi, Raees Khan, Seon-Woo Lee

HPLC analysis of the culture filtrate of wild-type SL341 and SL341D over time for the detection of homogentisate (HGA), an intermediate in pyomelanin synthesis. (A, B) Tyrosine standard and HGA standard peaks. (C, D) Culture filtrate analysis of SL341 after 48 and 72 h growth, respectively. (E, F) Culture filtrate analysis of SL341D after 48 and 72 h growth, respectively.

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