rspb20162598_si_001.pdf (1.37 MB)
Detailed method for phylogenetic reconstruction from Terrestrial reproduction as an adaptation to steep terrain in African toads
Version 2 2017-03-22, 13:44
Version 1 2017-03-13, 11:07
journal contribution
posted on 2017-03-22, 13:44 authored by H. Christoph Liedtke, Hendrik Müller, Julian Hafner, Johannes Penner, David J. Gower, Tomáš Mazuch, Mark-Oliver Rödel, Simon P. LoaderText detailing the molecular procedures undertaken to amplify genetic markers (including primer sequences and PCR conditions) and the bioinformatics workflow for generating alignments and performing phylogenetic reconstructions. Information on fossil calibration points is also given and a table of all sequences with GenBank Accession numbers used in this study.