ja5b12600_si_001.pdf (1.95 MB)
Design of Synthetic Polymer Nanoparticles That Facilitate Resolubilization and Refolding of Aggregated Positively Charged Lysozyme
journal contribution
posted on 2016-02-19, 00:00 authored by Masahiko Nakamoto, Tadashi Nonaka, Kenneth
J. Shea, Yoshiko Miura, Yu HoshinoDesigned polymer hydrogel nanoparticles
(NPs) capable of facilitating
resolubilization and refolding of an aggregated protein, positively
charged lysozyme, are prepared. NPs designed to interact strongly
with denatured lysozyme and relatively weakly with native lysozyme,
facilitated resolubilization and refolding of aggregated lysozyme.
Such NPs could be prepared by copolymerizing optimized combinations
and populations of functional monomers. The refolded lysozyme showed
native conformation and enzymatic activity. Eleven grams of aggregated
protein was refolded by 1 g of NPs. However, NPs having low affinity
to denatured lysozyme and NPs having high affinity to both denatured
and native lysozyme showed relatively low facilitation activity. Our
results suggest a potential strategy for the design of artificial
chaperones with high facilitating activity.