nl0155476_si_001.pdf (3.01 MB)
Deposition and Characterization of Extended Single-Stranded DNA Molecules on Surfaces
journal contribution
posted on 2001-06-15, 00:00 authored by Adam T. Woolley, Ryan T. KellyWe have devised an approach for generating well-extended, surface deposited, single-stranded DNA molecules having minimal intramolecular
base-pairing. This method depends on careful control of the mica surface charge, appropriate selection of the ionic strength of the DNA
solution, and controlled translation of a droplet of DNA solution along the surface. We have shown the general applicability of this technique
by elongating ΦX174, M13mp18, and λ single-stranded DNA on mica followed by imaging with atomic force microscopy. We have further
demonstrated the feasibility of more elaborate, multistep surface preparations by aligning λ double-stranded DNA and ΦX174 single-stranded
DNA in orthogonal directions on the same substrate.