Deletion of <i>BcBCK1</i>, <i>BcMKK1</i> or <i>BcBMP3</i> led to reduced mycelial growth, conidiation and melanin production in <i>B</i>. <i>cinerea</i>.

<p>(A) Colony morphology of 38B1, ΔBcBck1, ΔBcMkk1, ΔBcBmp3, ΔBcBck1-C, ΔBcMkk1-C and ΔBcBmp3-C on PDA after 3 and 10 days of incubation at 25°C. (B) Hyphae of ΔBcMkk1, but not ΔBcBck1 and ΔBcBmp3, were wavy. Bar = 100 μm. (C) Comparisons of the transcript levels of three melanin biosynthesis related genes <i>CMR1</i>, <i>SCD1</i> and <i>PKS13</i> among the above strains. The expression level of each gene in 38B1 was referred to 1. Values on the bars followed by the same letter are not significantly different at <i>P</i> = 0.05. (D) Comparisons in conidiation among the above strains on PDA plates after 10 days of incubation. Values on the bars followed by the same letter are not significantly different at <i>P</i> = 0.05.</p>