Degeneration of PC and molecular layer interneurons.

(a, b) Fluorescence images of the cerebellar cortex immunostained for parvalbumin from a 6-month-old wild-type mouse (WT) (a) and an age-matched SCA1 mouse (B05) (b). Black and white arrows show examples of PCs and interneurons, respectively. Scale bar, 50 μm. (c, d) Graphs showing the size of cell bodies of interneurons (WT; n = 110 cells from 3 mice, B05; n = 140 cells from 4 mice) (c) and of PCs (WT; n = 81 PCs from 3 mice, B05; n = 80 PCs from 4 mice) (d). Asterisks indicate a statistically significant difference between the wild-type mice and SCA1 mice; ***p<0.001 by unpaired t-test.