Decreased NK1.1pos iNKT cell numbers in the peripheral organs of T cell-specific Gfi1-deficient mice.

(A) The results of the flow cytometric analyses of the NK1.1 expression on the WT and Gfi1 KO iNKT cells in the lung, liver and spleen. (B) The absolute number with the standard deviation of NK1.1pos and NK1.1neg iNKT cells in the lung, liver and spleen of WT and Gfi1 KO mice (n = 5 for each group). (C) The results of the quantitative RT-PCR analysis of the Gfi1 mRNA expression in the splenic iNKT cells. The splenic iNKT cells were divided into four populations according to the expression of CD4 and NK1.1. The results are presented relative to the mRNA expression of 18s ribosomal RNA with the standard deviation (n = 3). (D) The results of the flow cytometric analyses of the CD4 and NK1.1 expression on WT and Gfi1 KO iNKT cells in the lung, liver and spleen. (E) The absolute number with the standard deviation of CD4negNK1.1neg, CD4negNK1.1pos, CD4posNK1.1neg and CD4posNK1.1pos iNKT cells in the lung, liver and spleen of WT and Gfi1 KO mice (n = 5 for each group). NS; not significant, *P<0.05, **P<0.01 (Student’s t-test).