Decreased NK1.1<sup>pos</sup> iNKT cell numbers in the peripheral organs of T cell-specific <i>Gfi1</i>-deficient mice.

<p><b>(A)</b> The results of the flow cytometric analyses of the NK1.1 expression on the WT and <i>Gfi1</i> KO iNKT cells in the lung, liver and spleen. <b>(B)</b> The absolute number with the standard deviation of NK1.1<sup>pos</sup> and NK1.1<sup>neg</sup> iNKT cells in the lung, liver and spleen of WT and <i>Gfi1</i> KO mice (n = 5 for each group). <b>(C)</b> The results of the quantitative RT-PCR analysis of the <i>Gfi1</i> mRNA expression in the splenic iNKT cells. The splenic iNKT cells were divided into four populations according to the expression of CD4 and NK1.1. The results are presented relative to the mRNA expression of <i>18s</i> ribosomal RNA with the standard deviation (n = 3). <b>(D)</b> The results of the flow cytometric analyses of the CD4 and NK1.1 expression on WT and <i>Gfi1</i> KO iNKT cells in the lung, liver and spleen. <b>(E)</b> The absolute number with the standard deviation of CD4<sup>neg</sup>NK1.1<sup>neg</sup>, CD4<sup>neg</sup>NK1.1<sup>pos</sup>, CD4<sup>pos</sup>NK1.1<sup>neg</sup> and CD4<sup>pos</sup>NK1.1<sup>pos</sup> iNKT cells in the lung, liver and spleen of WT and <i>Gfi1</i> KO mice (n = 5 for each group). NS; not significant, *P<0.05, **P<0.01 (Student’s <i>t</i>-test).</p>