1752-0509-1-9-1.jpg (28.55 kB)

Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility-0

figure

posted on 2011-12-31, 03:54 authored by Sourabh Kharait, Sampsa Hautaniemi, Shan Wu, Akihiro Iwabu, Douglas A Lauffenburger, Alan Wells

Copyright information:

Taken from "Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility"

BMC Systems Biology 2007;1():9-9.

Published online 29 Jan 2007

PMCID:PMC1839898.

ere grown on these surfaces for 24 hours in complete growth medium and quiesced for another 24 hours in medium containing 0.5% dialyzed FBS. EGF was added for a period of 1 hour, cells washed once with PBS and lysed. Cell lysates were resolved using SDS-PAGE and immunoblotted using specific antibodies for various phosphorylated proteins. At least 5 replicates for each signaling protein were created for polynomial modeling. Actin served as a loading control.