Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility-0
2011-12-31T03:54:57Z (GMT) by
Taken from "Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility"
BMC Systems Biology 2007;1():9-9.
Published online 29 Jan 2007
PMCID:PMC1839898.ere grown on these surfaces for 24 hours in complete growth medium and quiesced for another 24 hours in medium containing 0.5% dialyzed FBS. EGF was added for a period of 1 hour, cells washed once with PBS and lysed. Cell lysates were resolved using SDS-PAGE and immunoblotted using specific antibodies for various phosphorylated proteins. At least 5 replicates for each signaling protein were created for polynomial modeling. Actin served as a loading control.