Supplementary Table S2.pdf (502.59 kB)
Dataset for: The role of PCNA as a scaffold protein in cellular signaling is functionally conserved between yeast and humans
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posted on 2018-06-21, 11:45 authored by Camilla Olaisen, Hans Fredrik Kvitvang, Sungmin Lee, Eivind Almaas, Per Bruheim, Finn Drabløs, Marit OtterleiProliferating cell nuclear antigen (PCNA), a member of the highly conserved DNA sliding clamp family, is an essential protein for cellular processes including DNA replication and repair. A large number of proteins from higher eukaryotes contain one of two PCNA-interacting motifs: PIP-box (PCNA interacting protein-box) and APIM (AlkB homologue 2 PCNA-interacting motif). APIM has been shown to be especially important during cellular stress. PIP-box is known to be functionally conserved in yeast, and here we show that this is also the case for APIM. Several of the 84 APIM-containing yeast proteins are associated with cellular signaling as hub-proteins, which are able to interact with a large number of other proteins. Cellular signaling is highly conserved throughout evolution, and we recently suggested a novel role for PCNA as a scaffold protein in cellular signaling in human cells. A cell-penetrating peptide containing the APIM sequence increases the sensitivity towards the chemotherapeutic agent cisplatin in both yeast and human cells, and both yeast and human cells become hypersensitive when the Hog1/p38 MAPK pathway is blocked. These results suggest that the interactions between APIM-containing signaling proteins and PCNA during the DNA damage response is evolutionary conserved between yeast and mammals, and that PCNA has a role in cellular signaling also in yeast.
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Categories
- Regenerative medicine (incl. stem cells)
- Synthetic biology
- Plant cell and molecular biology
- Animal cell and molecular biology
- Evolution of developmental systems
- Structural biology (incl. macromolecular modelling)
- Proteomics and intermolecular interactions (excl. medical proteomics)
- Evolutionary biology not elsewhere classified
- Signal transduction
- Cancer cell biology
- Systems biology
- Bioinformatics and computational biology not elsewhere classified
- Biostatistics
- Computational methods in fluid flow, heat and mass transfer (incl. computational fluid dynamics)
Keywords
PCNAphosphatidylinositolMAPKsignalingconservationDNA damage responsehypersensitivityStem CellsSynthetic BiologyMolecular BiologyDevelopmental BiologyStructural BiologyProteomics and Intermolecular Interactions (excl. Medical Proteomics)Evolutionary BiologySignal TransductionCancer Cell BiologySystems BiologyBioinformaticsBiostatisticsHeat and Mass Transfer Operations
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