Repeated electroacupuncture treatment attenuated hyperalgesia through suppression of spinal glial activation in chronic neuropathic pain rats.xls (57.5 kB)

Data on repeated electroacupuncture treatment for attenuated hyperalgesia through suppression of spinal glial activation in chronic neuropathic pain rats

dataset

posted on 2018-02-23, 10:28 authored by Jun-ying Wang, Yong-hui Gao, Li-na Qiao, Jian-liang Zhang, Duan-mu Cheng Ling, Ya-xia Yan, Shu-ping Chen, Jun-ling Liu

This dataset consists of a single Excel (.xls) file data containing data on rats grouped according to chronic constrictive injury (CCI) and electroacupuncture (EA) treatment, for 1) pain thresholds, 2) the cumulative analgesic effect of EA as influenced by astrocytes and microgliacytes and 3) the expression of glial fibrillary acidic protein (GFAP) of the lumbar spinal cord.

Methods

A total of 120 male Wistar rats were used in the study. The neuropathic pain model was established by chronic constrictive injury (CCI) of the sciatic nerve. The rats were randomly divided into sham group, CCI group, and sham CCI+EA group, and CCI+EA group. EA was applied to bilateral Zusanli (ST36) Yanlingquan (GB34). The mechanical (both time and force responses) and thermal pain thresholds (PTs) of the bilateral hind-paws were measured. The number of microgliacytes and activity of astrocytes in the dorsal horns (DHs) of lumbar spinal cord (L4-5) were examined by immunofluorescence staining, and the expression of glial fibrillary acidic protein (GFAP) protein was detected by western blot.

The related study consists of three sections:

The 1st section (see tab fig1) was designed to examine the effect of EA on pain thresholds of bilateral hind-paws. 36 animals in this part were randomly divided into 3 groups: sham CCI control, CCI model and EA (n=12 in each).

The 2nd section (see tab iba) was designed to observe different roles of astrocytes and microgliacytes in the cumulative analgesic effect of EA by using immunofluorescent staining. A total of 54 rats were randomized into 8 groups: normal control, sham CCI 6 day (D), sham CCI 18D, CCI 6D, CCI18D, sham CCI+EA2D, sham CCI+EA2 week(W), CCI+EA2D group, and CCI+EA2W, with 6 rats in each group.

The 3rd section (see tabs gfap & wb) was designed to observe the effect of EA on the expression of glial fibrillary acidic protein (GFAP) of the lumbar spinal cord. Thirty rats were randomized into 5 groups: normal control, model, CCI+EA2D, CCI+EA1W and CCI+EA2W (n=6 in each). Randomization scheme was created using the standard =RAND () function in Microsoft Excel.

Background to related study:

Repeated EA can relieve neuropathic pain and mirror-image pain in chronic neuropathic pain rats, which is probably associated with its effect in downregulating glial cell activation of the lumbar spinal cord, the microgliacyte first and astrocyte later.