Code_CRLB.zip (3.73 MB)
Cramér-Rao lower bound (CRLB) on lifetime estimates
Version 3 2019-07-17, 08:43
Version 2 2019-07-11, 09:12
Version 1 2019-06-28, 07:09
software
posted on 2019-07-17, 08:43 authored by Dorian Bouchet, Valentina Krachmalnicoff, Ignacio IzeddinIgnacio IzeddinThis MATLAB code computes the Cramer-Rao lower bound (CRLB) on the decay rate (or lifetime) estimates. It was developed by Dorian Bouchet (dorianf.bouchet@gmail.com) with inputs from Valentina Krachmalnicoff and Ignacio Izeddin. If you use it for your data analysis, thanks for citing the following research article:
D. Bouchet, V. Krachmalnicoff, and I. Izeddin, "Cramér-Rao analysis of lifetime estimations in time-resolved fluorescence microscopy," Opt. Express 27, 21239-21252 (2019)
The main script is "crlb_main.m". It is commented and it includes two examples. The main script calls the functions "crlb_parameters.m" used to properly normalize input parameters, "crlb_coefficients.m" used to compute the exponential decays and their derivatives, and "crlb_fisher.m" used to compute and invert the information matrix. The files "background.mat" and "irf.mat" contain experimentally measured backgound noise and IRF, and can be imported from the main script.
D. Bouchet, V. Krachmalnicoff, and I. Izeddin, "Cramér-Rao analysis of lifetime estimations in time-resolved fluorescence microscopy," Opt. Express 27, 21239-21252 (2019)
The main script is "crlb_main.m". It is commented and it includes two examples. The main script calls the functions "crlb_parameters.m" used to properly normalize input parameters, "crlb_coefficients.m" used to compute the exponential decays and their derivatives, and "crlb_fisher.m" used to compute and invert the information matrix. The files "background.mat" and "irf.mat" contain experimentally measured backgound noise and IRF, and can be imported from the main script.
