Consumption of H₂S from Our Daily Diet: Determination by a Simple Chemosensing Method

A unique method has been developed for comparative analysis of H₂S produced from food samples from our daily diet, both qualitatively and quantitatively. The selective detection of H₂S has been executed by introducing a simple chemodosimeter (PN-N₃) that gives response on the basis of intramolecular charge transfer. UV–vis, fluorimetric, and NMR titrations were performed to demonstrate the sensing mechanism and electronic environment of PN-N₃ in the presence of H₂S. Density functional theory calculations were performed to validate the mechanism of azide (PN-N₃) reduction to amine (PN-NH₂) by the strong reducing power of H₂S. The potentiality of this chemosensing method is that it could be treated as a simple, less-time-consuming, and cost-effective method for determining H₂S in biological samples in the nanomolar range.