Conditional Ssb1-deleted mice are sensitive to IR.
(A) Schematic diagram of the radiation challenge assay in Ssb1 deleted mice. Rosa26-CreERT2: Ssb1+/+, Rosa26-CreERT2: Ssb1+/− and Rosa26-CreERT2: Ssb1−/− mice were challenged with 8 Gy of total body irradiation (TBI). The acute lethal response of mice to TBI was evaluated over a 30-day observation period. (B) Kaplan-Meier survival analysis of irradiated mice. Kaplan-Meier survival curves compared by log-rank (Mantel-Cox) analysis showed significant difference between Rosa26-CreERT2: Ssb1−/− mice and the other two groups (**P<0.01), while no difference was found between Rosa26-CreERT2: Ssb1+/+ and Rosa26-CreERT2: Ssb1+/− groups. (C) Representative images of Haematoxylin and eosin, Ki67 (cell proliferation) and ApopTag (cell death) staining on small intestine sections from mice at Day 5 post 8 Gy of TBI.