Figure_6.tif (2.51 MB)

Conditional Ssb1-deleted mice are sensitive to IR.

figure

posted on 2013-02-07, 00:50 authored by Wei Shi, Amanda L. Bain, Bjoern Schwer, Fares Al-Ejeh, Corey Smith, Lee Wong, Hua Chai, Mariska S. Miranda, Uda Ho, Makoto Kawaguchi, Yutaka Miura, John W. Finnie, Meaghan Wall, Jörg Heierhorst, Carol Wicking, Kevin J. Spring, Frederick W. Alt, Kum Kum Khanna

(A) Schematic diagram of the radiation challenge assay in Ssb1 deleted mice. Rosa26-CreER^T2: Ssb1^+/+, Rosa26-CreER^T2: Ssb1^+/− and Rosa26-CreER^T2: Ssb1^−/− mice were challenged with 8 Gy of total body irradiation (TBI). The acute lethal response of mice to TBI was evaluated over a 30-day observation period. (B) Kaplan-Meier survival analysis of irradiated mice. Kaplan-Meier survival curves compared by log-rank (Mantel-Cox) analysis showed significant difference between Rosa26-CreER^T2: Ssb1^−/− mice and the other two groups (**P<0.01), while no difference was found between Rosa26-CreER^T2: Ssb1^+/+ and Rosa26-CreER^T2: Ssb1^+/− groups. (C) Representative images of Haematoxylin and eosin, Ki67 (cell proliferation) and ApopTag (cell death) staining on small intestine sections from mice at Day 5 post 8 Gy of TBI.