Conditional IL-15 knockout in Ccl19-expressing cells leads to slightly decreased maintenance of inflationary T cells.
2018-04-13T17:46:01Z (GMT) by
<p>(A) Naïve C57BL/6 mice were adoptively transferred with naïve Maxi CD8 T cells one day prior to MCMV infection. Maxi T<sub>EM</sub> cells were sorted from lungs of latently infected donor mice and adoptively transferred into the conditional IL-15 knockout animals. Recovery of Maxi T cells was assessed in the lungs of recipients four weeks post transfer. Percentage of recovered Maxi cells in the lungs normalised to Cre-negative recipients is shown for <i>Ccl19</i>-Cre x IL-15<sup><i>fl/fl</i></sup> (B) mice as mean + SEM of n = 15–16 mice pooled from three independent experiments. Percentage of recovered Maxi cells in the lungs normalised to Cre-negative recipients is shown in CD11c-Cre <i>Il15</i><sup><i>fl</i>/f<i>l</i></sup> (C) and VE-Cadherin-Cre <i>Il15</i><sup><i>fl</i>/f<i>l</i></sup> (D) mice as mean + SEM of n = 7–15 mice pooled from two independent experiments. (B-D) ns, not significant (p>0.20); Statistical analyses were performed using the unpaired, two-tailed Student's <i>t</i> test (B-C) or the non-parametric Mann-Whitney <i>U</i> test (D).</p>