Conditional IL-15 knockout in Ccl19-expressing cells leads to slightly decreased maintenance of inflationary T cells.
(A) Naïve C57BL/6 mice were adoptively transferred with naïve Maxi CD8 T cells one day prior to MCMV infection. Maxi TEM cells were sorted from lungs of latently infected donor mice and adoptively transferred into the conditional IL-15 knockout animals. Recovery of Maxi T cells was assessed in the lungs of recipients four weeks post transfer. Percentage of recovered Maxi cells in the lungs normalised to Cre-negative recipients is shown for Ccl19-Cre x IL-15fl/fl (B) mice as mean + SEM of n = 15–16 mice pooled from three independent experiments. Percentage of recovered Maxi cells in the lungs normalised to Cre-negative recipients is shown in CD11c-Cre Il15fl/fl (C) and VE-Cadherin-Cre Il15fl/fl (D) mice as mean + SEM of n = 7–15 mice pooled from two independent experiments. (B-D) ns, not significant (p>0.20); Statistical analyses were performed using the unpaired, two-tailed Student's t test (B-C) or the non-parametric Mann-Whitney U test (D).