Concomitant loss of FoxO and high TORC1 activity causes precocious differentiation under NR.

(A) Pigmentation in eye discs with Tsc1 and FoxO co-knockdown (disc is marked by dashed-red line, still attached to mouth hook) dissected from larvae reared under NR. Scale bar is 0.5 mm. (B-D) Autofluorescence seen in the green and blue (B’-D’) channels for (B”) cuticle (marked by arrow), (C”) intracellular autofluorescence (marked by arrowhead) and (D”) extracellular autofluorescence (marked by asterisks). Scale bars are 75 μm. Quantification of percentage of discs with (E) pigmentation, (F) cuticle, (G) intracellular autofluorescence, and (H) extracellular autofluorescence. n = 6–10 for control and FoxO knockdown, and n > 30 for the remaining cases.