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Complementation of non-photosynthetic phenotype of by mutant tRNA
figure
posted on 2011-12-30, 15:04 authored by Shinya Murakami, Katrin Kuehnle, David B. SternCopyright information:
Taken from "A spontaneous tRNA suppressor of a mutation in the nuclear gene required for stability of the chloroplast mRNA"
Nucleic Acids Research 2005;33(10):3372-3380.
Published online 9 Jun 2005
PMCID:PMC1148173.
© The Author 2005. Published by Oxford University Press. All rights reserved
() Schematic diagram of the constructs. Genomic DNA fragments including WT or tRNA (CGA)-1 were inserted into a position preceding the cassette of pMS171. () Cells with the indicated amount of chlorophyll were spotted on TAP or minimal medium and incubated for 8 days in the light.