Comparisons of IL-10 and transforming growth factor (TGF)-β production by mononuclear lymphocytes from lymphoid organs of tolerized and nontolerized mice

<p><b>Copyright information:</b></p><p>Taken from "Induction of IL-10-producing CD4CD25T cells in animal model of collagen-induced arthritis by oral administration of type II collagen"</p><p>Arthritis Research & Therapy 2004;6(3):R213-R219.</p><p>Published online 11 Mar 2004</p><p>PMCID:PMC416445.</p><p>Copyright © 2004 Min et al., licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.</p> Organs were extracted from normal DBA/1, and tolerized and nontolerized collagen-induced arthritis (CIA) mice at 5 weeks after primary immunization with type II collagen (CII) for induction of CIA. Mononuclear cells were isolated from the Peyer's patch and the spleen, and production of IL-10 and TGF-β was assessed by sandwich ELISA analyses of culture supernatants. The data represent average values from three independent measurements.