Comparison of translational suppression by HSV-1 and HSV-2 during cell-cell spread.

(A) Cells were infected (MOI 10) with HSV-1[KOS] or HSV-2[186] according to the standard workflow in Fig 1b, analysed for newly synthesised protein (green) or VP5 accumulation (red). The merged panel also includes the corresponding phase images of the same fields. (B) Quantitative analysis of translation at the single cell level based on HPG intensity (green) in zones demarcated by the translational shutoff. Green dots indicate uninfected cells beyond the shutoff zone, black squares indicate those cell within a contiguous zone exhibiting shutoff and orange triangles indicate more central cells within the plaque, positive for VP5 and translational recovery.