Fig 3.TIF (1.33 MB)

Comparison of effect of compounds on hippocampal fEPSPs.

figure

posted on 2016-06-17, 05:21 authored by Jason Wallach, Heather Kang, Tristan Colestock, Hamilton Morris, Zuner A. Bortolotto, Graham L. Collingridge, David Lodge, Adam L. Halberstadt, Simon D. Brandt, Adeboye Adejare

A & B. Graphs showing basic method for studying effect of compounds on NMDAR-fEPSPs in CA1 region comparing effects of 10 μM D-AP5 (A) and DPH (B). After 30 min baseline recording (a), AMPA and GABA receptor mediated events were blocked pharmacologically (b), the stimulus strength increased and a new baseline was obtained for the NMDAR-fEPSP (c) before administering the test compound for 3 h (d). Note firstly the difference in shape between the AMPA receptor-mediated (a) and the NMDA receptor-mediated (c) fEPSPs, and secondly the slow onset of the pharmacological block by DPH (B) compared with D-AP5 (A). C. AMPA receptor-mediated fEPSPs (a) were not reduced by 50/100 μM 2-MXP (b) but the remaining NMDAR-fEPSPs after AMPA and GABA receptor antagonism were abolished in the presence of 10 μM 2-MXP(c). D. Superimposed single exponentials, showing time course of reduction of NMDAR-fEPSP by 1 μM test compounds superfused for up to 12 h.