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Comparative expression analysis of starch degrading genes between dormant and non-dormant wheat seeds

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posted on 2017-12-06, 20:46 authored by Menghan Sun, Yuji Yamasaki, Belay T. Ayele

To gain insights into the molecular basis of starch degradation in wheat seeds with respect to dormancy maintenance and release, this study compared the expression of starch degrading genes between dormant and after-ripened seeds in both dry and imbibed states. Furthermore, the study examined the effect of ABA on the expression of starch degrading genes during imbibition of non-dormant seeds. Release of dormancy due to after-ripening led to the upregulation of specific genes encoding α-amylase and α-glucosidase during imbibition while dormancy maintenance is associated with repression of these genes. It appears from our result that ABA delays the germination of wheat seeds at least partly through repression of the starch degrading genes.

Funding

This work was supported by a grant from the Natural Sciences and Engineering Research Council of Canada, Western Grains Research Foundation, and Manitoba Wheat and Barley Growers Association to BTA.

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