Combined oseltamivir plus sirolimus treatment reduced mTOR-NF-κB-NLRP3 inflammasome-IL-1β axis in lung epithelial cells.

Infected MLE12 cells were harvested and analyzed for protein expression by western blotting. (A) Protein levels of p-mTOR, mTOR, p-S6K, and S6K. (B) Expression of p-mTOR relative to mTOR, p-S6K relative to S6K, and p-mTOR, mTOR, p-S6K, and S6K relative to β-actin. (C) Protein levels of NLRP3, caspase-1 p20, and pro-caspase-1. (D) Expression of NLRP3 and pro-caspase-1 relative to β-actin, and caspase-1 p20 relative to pro-caspase-1. (E) Protein levels of IL-1β and pro-IL-1β. (F) Expression of IL-1β and pro-IL-1β relative to β-actin. (G) Protein levels of p-p65, p65, p-IκB-α, and IκB-α. (H) Expression of p-p65 relative to p65, p-IκB-α relative to IκB-α, and p-p65, p65, p-IκB-α, and IκB-α relative to β-actin. Data are representative of two independent experiments and presented as mean ± SEM. *, **, and *** represent p < 0.05, 0.01, and 0.001, respectively.