Clusters Highlighted in Protein Structures.

A) PIK3CA (gray) bound to PIK3R1 (orange). PIK3CA has two clusters (539–547 in green and 1043–1049 in blue) with very different global gene expression association significance levels in Breast Cancer (BRCA) discussed in the text. B) Residues 30–40 of CTNNB1 (blue) bound to BTRC (gray). This region of Beta-catenin is inside the 25–45 cluster which contains degradation regulating phosphorylated amino acids and is strongly associated with global gene expression changes in uterine corpus endometrial carcinoma (UCEC) and liver hepatocellular carcinoma (LIHC). Bottom bars in both plots show linear protein sequences with additional clusters in dark gray and PFAM protein domains in light gray. Mutation count histograms are shown for specific tumor types above the sequence with green dots representing synonymous mutations, blue dots representing missense mutations, and yellow dots nonsense mutations. Protein images created using UCSF Chimera [26].