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Chemokine receptor expression by peripheral blood monocytes

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posted on 2011-12-30, 17:13 authored by Kenji Hayashida, Toshihiro Nanki, Hermann Girschick, Sule Yavuz, Takahiro Ochi, Peter E Lipsky

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Taken from "Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8"

Arthritis Research 2001;3(2):118-126.

Published online 5 Jan 2001

PMCID:PMC17828.

Copyright © 2001 BioMed Central Ltd on behalf of the copyright holder

Peripheral blood SRBC rosette negative cells (1 × 10) were stained with anti-CD14 mAb and various chemokine receptor mAbs, and were analyzed by flow cytometry. CD14 cells were gated, and chemokine receptor expression by the CD14 monocytes is shown (solid line). Dotted lines show staining by isotype-matched control mAb. Percentage of positive cells is also shown in the histograms. Staining was from one of nine experiments. FL-1 height, FITC fluorescence; FL-2 height, PE fluorescence.

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