AR-3-2-118-4.jpg (26.28 kB)

Chemokine production by various cell lines

figure

posted on 2011-12-30, 17:13 authored by Kenji Hayashida, Toshihiro Nanki, Hermann Girschick, Sule Yavuz, Takahiro Ochi, Peter E Lipsky

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Taken from "Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8"

Arthritis Research 2001;3(2):118-126.

Published online 5 Jan 2001

PMCID:PMC17828.

Production of MCP-1, IL-8, RANTES, IP-10, and MIP-1β was measured by ELISA. Various cell lines (1 × 10/ml) were cultured for 24 h with 2 ml DMEM containing 5% FBS, and supernatants were collected. The contents of MCP-1, IL-8, and RANTES are shown; MIP-1β and IP-10 were not detected (less than 10 pg/ml). RA6/1, RA8/3, and Sy77 are RA SCLs, OA5/26 is a SCL from OA, and FBHG is a skin fibroblast line. The mean and SEM were calculated from triplicates of one representative experiment out of three with similar results. Statistical analysis was performed with the Student test. < 0.05 and < 0.01.