AR-3-2-118-6.jpg (47.52 kB)

Chemokine production by RA SCL stimulated with TNF-α

figure

posted on 2011-12-30, 17:13 authored by Kenji Hayashida, Toshihiro Nanki, Hermann Girschick, Sule Yavuz, Takahiro Ochi, Peter E Lipsky

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Taken from "Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8"

Arthritis Research 2001;3(2):118-126.

Published online 5 Jan 2001

PMCID:PMC17828.

Production of MCP-1, IL-8, RANTES, IP-10, and MIP-1β was measured by ELISA. Various cell lines (1 × 10/ml) were cultured for 24 h in 2 ml DMEM containing 5% FBS with or without 2 ng/ml TNF-α, and supernatants were collected. The content of MCP-1, IL-8, RANTES and IP-10 are shown; MIP-1β was not detected (less than 10 pg/ml). RA6/1, RA8/3, and Sy77 are RA SCLs. The mean and SEM were calculated from triplicates of one representative experiment out of three with similar results. Statistical analysis was performed with the Student test. < 0.05 and < 0.01.