Chemical constituents and their bioactivities from the fruits of <i>Vitex negundo</i> var. <i>cannabifolia</i>

<p>Phytochemical investigation of the fruits of <i>Vitex negundo</i> var. <i>cannabifolia</i> led to the isolation of 22 compounds (<b>1</b>–<b>22</b>). Their structures were elucidated mainly by spectroscopic analysis and comparison with the literature data. Among them, compounds <b>1</b> and <b>2</b> were two new artificial lignans. Primary bioassay showed that the polymethoxyflavones <b>9</b>–<b>12</b> displayed moderate-to-weak cytotoxicity against human HepG2 and rat C6 cell lines, while the triterpenoids <b>13</b>–<b>17</b> exhibited significant brine shrimp lethality with LC<sub>50</sub>values of 7.5–29.4 μM.</p>