Chemical characterisation of the constituents of <i>Eugenia protenta</i> McVaugh and leishmanicidal activity of dimethylxanthoxylin

<p>The chemical study of <i>Eugenia protenta</i> McVaugh extracts performed by classical and high-performance liquid chromatography techniques and spectral methods has led to the identification of known triterpenoids, flavonoids and an acetophenone derivative (dimethylxanthoxylin). The effect of dimethylxanthoxylin on <i>Leishmania</i> (<i>Leishmania</i>) <i>amazonensis</i> was evaluated against the promastigotes forms after 96 h of treatment. Dimethylxanthoxylin reduced 57 and 59% of the promastigotes growth when treated with 50 and 100 μg/mL solutions, respectively (IC<sub>50</sub> 117.35 μg/mL or 52.3 μM). Cytotoxicity experiments using MTT assays showed that this substance did not promote cell death after 24 h of treatment. Dimethylxanthoxylin was active on the promastigotes and could be a promising agent for treating leishmaniasis.</p>