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Characterization of wild-type and S326C OGG1 expressed in human cells
figure
posted on 2011-12-30, 17:52 authored by Jeff W. Hill, Michele K. EvansCopyright information:
Taken from "Dimerization and opposite base-dependent catalytic impairment of polymorphic S326C OGG1 glycosylase"
Nucleic Acids Research 2006;34(5):1620-1632.
Published online 20 Mar 2006
PMCID:PMC1405821.
© The Author 2006. Published by Oxford University Press. All rights reserved
() Comparision of 8-oxoguanine glycosylase activites of HeLa nuclear extracts from cells transfected with pCMV vector (lane 1), or pCMV vector expressing N-terminally FLAG-tagged wild-type (lane 2) or S326C OGG1 (lane 3). Left inset; Anti-FLAG western blot of 2 µg of nuclear extract prepared from HeLa cells transfected with pCMV vector (lane 1), pCMV wt (lane 2) or PCMV S326C (lane 3). Right inset; actual data shown graphically in (A). (B) Anti-FLAG western blot of 4 µg of nuclear extract from cells transfected with pCMV vector (lanes 1 and 2), pCMV wt (lanes 3 and 4) or pCMV S326C (lanes 5 and 6). Nuclear extracts were incubated at 0°C overnight with (lanes 2, 4 and 6) and without (lanes 1, 3 and 5) 2 mM BM[PEO].